primary antibody mouse monoclonal iggik anti cas9 ab 7a9 3a3 (Cell Signaling Technology Inc)
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primary antibody mouse monoclonal iggik anti cas9 ab 7a9 3a3
Primary Antibody Mouse Monoclonal Iggik Anti Cas9 Ab 7a9 3a3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cas9+antibody/bio_rxiv__64898__2026__03__30__715406-183-12-20
Average 86 stars, based on 1 article reviews
Primary Antibody Mouse Monoclonal Iggik Anti Cas9 Ab 7a9 3a3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cas9+antibody/bio_rxiv__64898__2026__03__30__715406-183-12-20
Average 86 stars, based on 1 article reviews
primary antibody mouse monoclonal iggik anti cas9 ab 7a9 3a3 - by Bioz Stars,
2026-09
86/100 stars
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Over Expression:Article Title: Identification of TUBB3 as an immunotherapy target in lung cancer by genome wide in vivo CRISPR screening. Article Snippet: Recent development of immune checkpoint inhibitors has revolutionized cancer immunotherapy.. Although these drugs show dramatic effects on a subset of cancer patients, many other tumors are non-responsive and the pathological mechanism of the resistance is largely unknown.. To identify genes underlying anti-PD-1 immunotherapy resistance using a systematic approach, we performed an in vivo genome wide CRISPR screening in lung cancer cells. Article Title: Identification of TUBB3 as an immunotherapy target in lung cancer by genome wide in vivo CRISPR screening Article Snippet: Cells after lentiviral infection were selected by 10 μg/mL Blasticidin (Thermo Fisher, R21001). .. CAS9 overexpression was confirmed by WB using Western Blot:Article Title: Identification of TUBB3 as an immunotherapy target in lung cancer by genome wide in vivo CRISPR screening. Article Snippet: Recent development of immune checkpoint inhibitors has revolutionized cancer immunotherapy.. Although these drugs show dramatic effects on a subset of cancer patients, many other tumors are non-responsive and the pathological mechanism of the resistance is largely unknown.. To identify genes underlying anti-PD-1 immunotherapy resistance using a systematic approach, we performed an in vivo genome wide CRISPR screening in lung cancer cells. Article Title: Vector-free intra-airway in vivo epigenetic editing. Article Snippet: .. The membranes were blocked with 5% non-fat milk and followed by immunoblotting with anti-His-tag antibody (Cell Signaling Technologies #2366 or Santa-Cruz Bio #sc-57598), Article Title: Identification of TUBB3 as an immunotherapy target in lung cancer by genome wide in vivo CRISPR screening Article Snippet: Cells after lentiviral infection were selected by 10 μg/mL Blasticidin (Thermo Fisher, R21001). .. CAS9 overexpression was confirmed by WB using Incubation:Article Title: In vivo prime editing rescues photoreceptor degeneration in nonsense mutant retinitis pigmentosa. Article Snippet: .. The retinal sections were incubated in blocking buffer for 1 h at room temperature and Nature Communications | (2025) 16:2394 12 then incubated with primary anti-rhodopsin antibody (Abcam, Car# ab221664,1:500) or Article Title: Ligand-modified rAAV6 vectors with nanoblades allow high-level gene knockin in HSPCs without compromising cell survival Article Snippet: The following day, the wells were incubated with washing buffer (phosphate-buffered saline [PBS]/0.05% Tween) and blocked with PBS/0.05%Tween/3%BSA (Sigma). .. Subsequently the wells were washed and the primary Article Title: In vivo prime editing rescues photoreceptor degeneration in nonsense mutant retinitis pigmentosa Article Snippet: .. The retinal sections were incubated in blocking buffer for 1 h at room temperature and then incubated with primary anti-rhodopsin antibody (Abcam, Car# ab221664,1:500) or Article Title: Ligand-modified rAAV6 vectors with nanoblades allow high level gene knock-in in HSPCs without compromising cell survival Article Snippet: The dilutions were performed in coating buffer (1% Triton) and were then coated onto 96-well-plates by incubation overnight at 4 C. The following day, the wells were incubated with washing buffer (phosphate-buffered saline [PBS]/0.05% Tween) and blocked with PBS/ 0.05%Tween/3%BSA (Sigma). .. Subsequently the wells were washed and the primary Blocking Assay:Article Title: In vivo prime editing rescues photoreceptor degeneration in nonsense mutant retinitis pigmentosa. Article Snippet: .. The retinal sections were incubated in blocking buffer for 1 h at room temperature and Nature Communications | (2025) 16:2394 12 then incubated with primary anti-rhodopsin antibody (Abcam, Car# ab221664,1:500) or Article Title: In vivo prime editing rescues photoreceptor degeneration in nonsense mutant retinitis pigmentosa Article Snippet: .. The retinal sections were incubated in blocking buffer for 1 h at room temperature and then incubated with primary anti-rhodopsin antibody (Abcam, Car# ab221664,1:500) or Control:Article Title: Vector-free intra-airway in vivo epigenetic editing. Article Snippet: .. The membranes were blocked with 5% non-fat milk and followed by immunoblotting with anti-His-tag antibody (Cell Signaling Technologies #2366 or Santa-Cruz Bio #sc-57598), other:Article Title: Stable Cas9 expression regulates cell growth by facilitating mTORC2 activation Article Snippet: |
![a , Affinity proteomics (AP) to identify protein-protein interactomes for different TruEditors. FLAG-tagged TruEditors were immunoprecipitated from HEK293 cells after 24 hours and interacting proteins were identified on a Orbitrap Eclipse Tribrid mass spectrometer. b , Pearson correlation of peptide spectral match (PSM) counts ( n = 2 - 3 biological replicates per TruEditor). c , Protein-protein interaction networks for TruEditors and their significant interactors (average PSM fold-change over Cas9 alone > 10 and P adj < 0.05). Edge thickness (average PSM) and color (significance analysis of interactome [SAINT] score ) correspond to the strength of the connection. Nodes are colored by Gene Ontology category. d , Ternary complex of the Cas9- BLM 2-194 TruEditor with AP-validated interactors TOP3A and RMI1 as predicted by AlphaFold3. Key BLM 2-194 interaction residues at the interface with TOP3A ( light grey ) and RMI1 ( medium grey ) are highlighted in pink . e , Co-immunoprecipitation of FLAG-tagged Cas9 only, Cas9- BLM 2-194 and different Cas9- BLM 2-194 mutants (N8A, F35A, N8A/F35A, ΔA2-L21, ΔA2-T41) in HEK293 cells. f , Precise editing rates (GFP-to-BFP editing) for Cas9 only, Cas9- BLM 2-194 and Cas9- BLM 2-194 mutants in HEK293-GFP cells as measured by flow cytometry ( n = 3 biological replicates).](https://bio-rxiv-images-cdn.bioz.com/dois_ending_with_75/10__64898_slash_2026__02__19__706875/10__64898_slash_2026__02__19__706875___F4.large.jpg)